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dc.contributor.authorWendell, Douglas L.
dc.date.accessioned2017-09-06T16:53:31Z
dc.date.available2017-09-06T16:53:31Z
dc.date.issued2016-08-22
dc.identifier.citationWendell DL, Vaziri A, Shergill G (2016) The Gene Encoding Dihydroflavonol 4-Reductase Is a Candidate for the anthocyaninless Locus of Rapid Cycling Brassica rapa (Fast Plants Type). PLoS ONE 11(8): e0161394. doi:10.1371/journal.pone.0161394en_US
dc.identifier.urihttp://hdl.handle.net/10323/4581
dc.description.abstractRapid cycling Brassica rapa, also known as Wisconsin Fast Plants, are a widely used organism in both K-12 and college science education. They are an excellent system for genetics laboratory instruction because it is very easy to conduct genetic crosses with this organism, there are numerous seed stocks with variation in both Mendelian and quantitative traits, they have a short generation time, and there is a wealth of educational materials for instructors using them. Their main deficiency for genetics education is that none of the genetic variation in RCBr has yet been characterized at the molecular level. Here we present the first molecular characterization of a gene responsible for a trait in Fast Plants. The trait under study is purple/nonpurple variation due to the anthocyaninless locus, which is one of the Mendelian traits most frequently used for genetics education with this organism. We present evidence that the DFR gene, which encodes dihyroflavonol 4-reductase, is the candidate gene for the anthocyaninless (ANL) locus in RCBr. DFR shows complete linkage with ANL in genetic crosses with a total of 948 informative chromosomes, and strains with the recessive nonpurple phenotype have a transposon-related insertion in the DFR which is predicted to disrupt gene function.en_US
dc.description.sponsorshipThis work was funded by an American Recovery and Reinvestment Act grant 5 RC1 RR030293-02 from the National Institutes of Health (https://www.nih.gov/), National Center for Research Resources, USA, and the Research Excellence Fund of Oakland University (https://wwwp.oakland.edu/cbr/research-excellence-program/). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Kresge OA Funden_US
dc.language.isoen_USen_US
dc.publisherPLOS ONEen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectBrassicaen_US
dc.subjectDNA-binding proteinsen_US
dc.subjectInsertion mutationen_US
dc.subjectSeedlingsen_US
dc.subjectGenetic locien_US
dc.subjectRepeated sequencesen_US
dc.subjectChromosomesen_US
dc.titleThe Gene Encoding Dihydroflavonol 4-Reductase Is a Candidate for the anthocyaninless Locus of Rapid Cycling Brassica rapa (Fast Plants Type)en_US
dc.typeArticleen_US


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