The effects of black cohosh on the regulation of estrogen receptor (ERα) and progesterone receptor (PR) in breast cancer cells

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dc.contributor.authorSzmyd, Monica
dc.contributor.authorLloyd, Victoria
dc.contributor.authorHallman, Kelly M.
dc.contributor.authorMladenovik, Viktoria
dc.contributor.authorMcKee, Christina
dc.contributor.authorMorse, Mia
dc.contributor.authorBedgood, Tyler
dc.contributor.authorDinda, Sumi
dc.date.accessioned2018-09-13T11:11:59Z
dc.date.available2018-09-13T11:11:59Z
dc.date.issued2018-01-18
dc.description.abstractThe North American plant Cimicifuga racemosa, also known as black cohosh (BC), is a herb that recently has gained attention for its hormonal effects. As the usage of hormone replacement therapy is declining due to its adverse effects in women with cancer, many are turning to herbal remedies like BC to treat menopausal symptoms. It is crucial to determine whether the effects of BC involve estrogen receptor-alpha (ERα). Previous studies from our laboratory have shown ERα to be a possible molecular target for BC. In this study, we examined the effects of BC (8% triterpene glycosides) alone and in combination with hormones and antihormones on the cellular viability, expression of ERα and progesterone receptor (PR)-A/B, and cytolocalization of ERα in ER (+) and PR-A/B (+) T-47D breast cancer cells. Cells were cultured and proteins were extracted and quantified. Western blot analysis revealed alterations in the expression of ERα and PR after treatment with BC (5–100 µM). BC induced a concentration-dependent decrease in ERα and PR protein levels when compared to the control. Image cytometric analysis with propidium iodide staining was used to enumerate changes in T-47D cell number and viability. A decrease in T-47D cell viability was observed upon treatment with 5–100 µM BC. The ideal concentration of BC (100 µM) was used in combination with hormones and antihormones in an effort to further understand the possible similarities between this compound and other known effectors of ERα and PR. After a 24-hour concomitant treatment with and/or in combination of BC, estradiol, ICI 182, 780, and Tamoxifen, downregulation of ERα and PR protein levels was observed. Delineating the role of BC in the regulation of ERα, PR, as well as its mechanisms of action, may be important in understanding the influence of BC on hormone receptors in breast cancer.en_US
dc.identifier.citationSzmyd, M., Lloyd, V., Hallman, K., Aleck, K., Mladenovik, V., McKee, C., . . . Dinda, S. (2018). The effects of black cohosh on the regulation of estrogen receptor (ERα) and progesterone receptor (PR) in breast cancer cells. Breast Cancer: Targets and Therapy, 10, 1-11. doi:10.2147/BCTT.S144865en_US
dc.identifier.urihttp://hdl.handle.net/10323/4758
dc.language.isoen_USen_US
dc.relation.journalBreast Cancer: Targets and Therapyen_US
dc.subjectBlack cohoshen_US
dc.subjectBreast canceren_US
dc.subjectEstrogen receptor alphaen_US
dc.subjectProgesterone receptoren_US
dc.subjectHormone replacement therapyen_US
dc.titleThe effects of black cohosh on the regulation of estrogen receptor (ERα) and progesterone receptor (PR) in breast cancer cellsen_US
dc.typeArticleen_US

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