Isolation and comparative analysis of potential stem/progenitor cells from different regions of human umbilical cord

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dc.contributor.authorBeeravolu, Naimisha
dc.contributor.authorKhan, Irfan
dc.contributor.authorMcKee, Christina
dc.contributor.authorDinda, Sumi
dc.contributor.authorThibodeau, Bryan
dc.contributor.authorWilson, George
dc.contributor.authorPerez-Cruet, Mick
dc.contributor.authorBahado-Singh, Ray
dc.contributor.authorChaudhry, G. Rasul
dc.date.accessioned2016-10-27T16:57:49Z
dc.date.available2016-10-27T16:57:49Z
dc.date.issued2016-10-27
dc.description.abstractHuman umbilical cord (hUC) blood and tissue are non-invasive sources of potential stem/progenitor cells with similar cell surface properties as bone marrow stromal cells (BMSCs). While they are limited in cord blood, they may be more abundant in hUC. However, the hUC is an anatomically complex organ and the potential of cells in various sites of the hUC has not been fully explored.We dissected the hUC into its discrete sites and isolated hUC cells from the cord placenta junction (CPJ), cord tissue (CT), and Wharton's jelly (WJ). Isolated cells displayed fibroblastoid morphology, and expressed CD29, CD44, CD73, CD90, and CD105, and showed evidence of differentiation into multiple lineages in vitro. They also expressed low levels of pluripotency genes, OCT4, NANOG, SOX2 and KLF4. Passaging markedly affected cell proliferation with concomitant decreases in the expression of pluripotency and other markers, and an increase in chondrogenic markers. Microarray analysis further revealed the differences in the gene expression of CPJ-, CT- and WJ-hUC cells. Five coding and five lncRNA genes were differentially expressed in low vs. high passage hUC cells. Only MAEL was expressed at high levels in both low and high passage CPJ-hUC cells. They displayed a greater proliferation limit and a higher degree of multi-lineage differentiation in vitro and warrant further investigation to determine their full differentiation capacity, and therapeutic and regenerative medicine potential.en_US
dc.identifier.urihttp://hdl.handle.net/10323/4360
dc.language.isoen_USen_US
dc.subjectUmbilical corden_US
dc.subjectPlacentaen_US
dc.subjectPeri-natal stem/progenitor cellsen_US
dc.subjectDifferentiationen_US
dc.subjectProliferationen_US
dc.titleIsolation and comparative analysis of potential stem/progenitor cells from different regions of human umbilical corden_US
dc.typeArticleen_US

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