Advances and challenges in stem cell culture

Simple item page
dc.contributor.authorMcKee, Christina
dc.contributor.authorChaudhry, G. Rasul
dc.date.accessioned2019-01-16T16:42:31Z
dc.date.available2019-01-16T16:42:31Z
dc.date.issued2017-07-27
dc.description.abstractStem cells (SCs) hold great promise for cell therapy, tissue engineering, and regenerative medicine as well as pharmaceutical and biotechnological applications. They have the capacity to self-renew and the ability to differentiate into specialized cell types depending upon their source of isolation. However, use of SCs for clinical applications requires a high quality and quantity of cells. This necessitates large-scale expansion of SCs followed by efficient and homogeneous differentiation into functional derivatives. Traditional methods for maintenance and expansion of cells rely on two-dimensional (2-D) culturing techniques using plastic culture plates and xenogenic media. These methods provide limited expansion and cells tend to lose clonal and differentiation capacity upon long-term passaging. Recently, new approaches for the expansion of SCs have emphasized three-dimensional (3-D) cell growth to mimic the in vivo environment. This review provides a comprehensive compendium of recent advancements in culturing SCs using 2-D and 3-D techniques involving spheroids, biomaterials, and bioreactors. In addition, potential challenges to achieve billion-fold expansion of cells are discussed.en_US
dc.description.sponsorshipOakland University, Oakland University William Beaumont Institute for Stem Cell and Regenerative Medicine (OU-WB ISCRM), Michigan Head and Spine Institute (MHSI). C. McKee was supported by the Oakland University Provost Graduate Student Research Award. The article received publishing support from the Kresge OA fund.en_US
dc.identifier.citationMckee, C., & Chaudhry, G. (2017). Advances and challenges in stem cell culture. Colloids and Surfaces B: Biointerfaces, 159, 62–77. https://doi.org/10.1016/j.colsurfb.2017.07.051en_US
dc.identifier.urihttp://hdl.handle.net/10323/6491
dc.language.isoen_USen_US
dc.relation.journalColloids and Surfaces B: Biointerfacesen_US
dc.subject3-D cultureen_US
dc.subjectSelf-renewalen_US
dc.subjectBiomimickingen_US
dc.subjectLarge-scale expansionen_US
dc.subjectSelf-assembling scaffolden_US
dc.subjectHydrogelen_US
dc.subjectBioreactoren_US
dc.titleAdvances and challenges in stem cell cultureen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Advances and challenges in stem cell culture.pdf
Size:
3.69 MB
Format:
Adobe Portable Document Format
Description:
Main Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.41 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Biological Sciences Faculty Scholarship