The Interplay of Cholesterol and Glucose on Wound Healing
Heart disease has long been on the rise in the United States, bringing with it a host of comorbidities and additional complications. To combat these developments, physicians may prescribe a statin drug to lower a patient’s cholesterol; a key component of both heart disease and cell membrane formation during wound healing. The goal of this project is to determine the role of statin treatment in the wound healing process in human dermal fibroblasts. The migration of cells to the wound environment will be observed with and without the conditions of elevated glucose, elevated cholesterol, and application of statin treatment. Cell migration will be evaluated through the monitoring of the enzyme deubiquitinase USP47 and its substrate β-catenin. These results will provide a more complete idea of the factors that prevent wound healing and contribute to chronic wound development, such as those found in diabetics, as well as give a starting point for potential treatments for these wounds. In this experiment it is hypothesized that compared to the wells not given any treatment, the wells treated with statins and glucose will show decreased cell migration and proliferation, as well as decreased levels of USP47 and β-catenin; and wells treated with LDL (low density lipoprotein) cholesterol will show increased cell migration and proliferation and increased protein levels.
Human Dermal Fibroblasts
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